产品简介
GLuc-ON™ 启动子报告克隆在GLuc报告基因上游插入一段1.2~1.5 kb的序列，这段插入序列与基因转录起始位点（TSS）上游大约1.5kb到下游200bp之间的5’侧翼序列一致，可以通过观察荧光素酶的活性来研究启动子对基因的调节作用。
GeneCopoeia可提供预制和定制的GLuc-ON™ 启动子克隆，包括单报告基因载体系统和双报告基因载体系统。单报告基因载体系统以Gluc、mCherry或GFP作为启动子的报告基因；双报告基因载体系统以Gluc作为启动子报告基因，以分泌型碱性磷酸酶 （SEAP）作为信号标准化的内参，可在活细胞中对超过20,000种人和18,000种小鼠启动子进行实时活性分析。
图1. GLuc-ON™ 启动子报告克隆的双报告基因检测原理图。
优势 |
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活细胞分析
- 分泌型Gluc报告子
- 无需裂解细胞
- 节省样本，减少突变，简化试验流程（如脉冲追踪分析等）
实时研究
- 可获得即时检测数据
- 与实时活性分析过程类似
分泌型双报告系统
- 分泌型Gluc和分泌型碱性磷酸酶
- 对多样本常规转染进行精确比较
高通量兼容
- 可用于信号通路研究
- 可进行高通量研究
高灵敏度
- GLuc比萤火虫或Renilla荧光素酶灵敏度高1000倍
使用方便
- 所有启动子报告克隆均可直接用于细胞转染
Gaussia 荧光素酶
GLuc-ON™ 启动子克隆采用经过修饰的GLuc（mGLuc）作为报告基因，能产生强烈且稳定的荧光信号，克服了人野生型GLuc（wtGLuc）信号衰退快的缺点。
图2. mGLuc (蓝色) 和 wtGLuc (红色)的信号稳定性比较。左侧：含有稳定剂的缓冲液；右侧：常规缓冲液。
双报告系统
GLuc-ON™ 启动子克隆可以将分泌型碱性磷酸酶（SEAP）作为第二个报告子和内参。这种双报告系统可以对多样本常规转染进行精确比较。
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图3. 不同启动子在H1B1B和HEK293T细胞中的活性分析。双报告系统启动子克隆和对照以两个重复转染进两种细胞中。转染24小时（(HEK293T）和48小时（H1B1B）后分析样品。NEG（包含非启动子序列）和EMPTY（载体不含启动子）作为阴性对照。 |
载体和对照
载体类型 |
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| 载体 | 报告基因 | 内参基因 | 筛选标记 | 载体类型 |
| pEZX-PG04 | Gaussia luciferase (GLuc) | 分泌型碱性磷酸酶(SEAP) | Puromycin | 非病毒 |
| pEZX-PG02 | Gaussia luciferase (GLuc) | N/A* | Puromycin | 非病毒 |
| pEZX-PF02 | eGFP | N/A* | Puromycin | 非病毒 |
| pEZX-PM02 | mCherry | N/A* | Puromycin | 非病毒 |
| pEZX-PL01 | Firefly luciferase | Renilla luciferase | Puromycin | 非病毒 |
| pEZX-LvPG04 | Gaussia luciferase (GLuc) | 分泌型碱性磷酸酶((SEAP) | Puromycin | 慢病毒 |
| pEZX-LvPG02 | Gaussia luciferase (GLuc) | N/A* | Puromycin | 慢病毒 |
| pEZX-LvPF02 | eGFP | N/A* | Puromycin | 慢病毒 |
| pEZX-LvPM02 | mCherry | N/A* | Puromycin | 慢病毒 |
| pEZX-LvPM03 | tdTomato | N/A* | Puromycin | 慢病毒 |
| pEZX-LvPL01 | Firefly luciferase | Renilla luciferase | Puromycin | 慢病毒 |
* SEAP可以在分泌型载体中表达。
对照克隆和SEAP表达克隆
Tear Away License
订购
预制的启动子报告克隆GeneCopoeia提供超过20,000种人和18,000种小鼠不同载体的预制启动子报告克隆。
慢病毒载体的启动子报告克隆GeneCopoeia提供客户定制的慢病毒载体启动子报告克隆。 了解慢病毒载体价格信息，请查询。
定制启动子报告克隆 |
需要定制克隆来比较启动子不同长度或不同区域的功能，请发邮件到 support@igenebio.com 或拨打4006-020-200
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| GLuc-ON™ Promoter Reporter Clones Cancellation, QA, License, and Warranty |
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已发表文章
- Yu, C., et al. (2023). Flot2 acts as a novel mediator of podocyte injury in proteinuric kidney disease. Int J Biol Sci doi: 10.7150/ijbs.78945 [OMICSLINK™ empty vector; GLuc-ON™ promoter reporter clone; Lenti-Pac™ HIV Expression Packing Kit; Secrete-Pair™ Dual Luminescence Assay Kit]
- Zhang, Y., et al. (2023). ZNF451 favors triple-negative breast cancer progression by enhancing SLUG-mediated CCL5 transcriptional expression. Cell reports doi: 10.1016/j.celrep.2023.112654 [GLuc-ON™ promoter-reporter clone; Secrete-Pair™ Luciferase Assay Kit]
- Quintanal-Villalonga, A., et al. (2023). Exportin 1 inhibition prevents neuroendocrine transformation through SOX2 down-regulation in lung and prostate cancers. SCIENCE TRANSLATIONAL MEDICINE doi: 10.1126/scitranslmed.adf7006 [GLuc-ON™ promoter-reporter clone; OMICSLINK™ ORF expression control clones]
- Yang, Y.C., et al. (2023). The RNA-binding protein KSRP aggravates malignant progression of clear cell renal cell carcinoma through transcriptional inhibition and post-transcriptional destabilization of the NEDD4L ubiquitin ligase. J Biomed Sci doi: 10.1186/s12929-023-00949-9 [GLuc-ON™ promoter-reporter clone; Secrete-Pair™ Gaussia Luciferase Assay kit]
- Morelli, E., et al. (2023). A MIR17HG-derived long noncoding RNA provides an essential chromatin scaffold for protein interaction and myeloma growth. Blood doi: 10.1182/blood.2022016892 [GLuc-ON™ Promoter Reporter Vector]
- Kalantari, P., et al. (2023). The balance between gasdermin D and STING signaling shapes the severity of schistosome immunopathology. PNAS doi: 10.1073/pnas.2211047120 [GLuc-ON™ Promoter Reporter Vector]
- Pflug, K.M., et al. (2023). Transcriptional induction of NF-κB-inducing kinase by E2F4/5 facilitates collective invasion of GBM cells. Sci Rep doi: 10.1038/s41598-023-38996-9 [GLuc-ON™ NIK promoter-reporter clone]
- Li, Q., et al. (2023). Suppression of microglial Ccl2 reduces neuropathic pain associated with chronic spinal compression. Front Immunol doi: 10.3389/fimmu.2023.1191188 [GLuc-ON™ TMEM119 promoter-reporter clone]
- Chen, H., et al. (2023). PLAGL2 promotes bladder cancer progression via RACGAP1/RhoA GTPase/YAP1 signaling. Cell Death Dis doi: 10.1038/s41419-023-05970-2 [GLuc-ON™ NNAT promoter-reporter clone]
- Plasterer, C., et al. (2023). NNAT is a novel mediator of oxidative stress that suppresses ER + breast cancer. Mol Med doi: 10.1186/s10020-023-00673-y [GLuc-ON™ NNAT promoter-reporter clone; OMICSLINK™ NRF1，E2F1 and E2F4 ORF expression clone]
- Yang, X., et al. (2023). Netrin-1 controls inflammation in response to ischemic stroke through altering microglia phenotype. Front Immunol doi: 10.3389/fimmu.2023.1178638 [GLuc-ON™ TMEM119 promoter reporter clone]
- Lee, K-H., et al. (2023). New oncogenic functions of LINE1 retroelement as a ceRNA for tumor suppressive microRNA miR-126 on ENPP5. PLoS One doi: 10.1371/journal.pone.0286814 [Secrete-Pair™ Gaussia Luciferase reporter vector]
- Xiong, X., et al. (2023). E2F1‑mediated RAB34 upregulation accelerates the proliferation and inhibits the cell cycle arrest and apoptosis of acute myeloid leukemia cells. Exp Ther Med doi: 10.3892/etm.2023.12088 [GLuc-ON™ RAB34 promoter-reporter clone]
- Cruz, M.A., et al. (2023). Micronutrient optimization for tissue engineered articular cartilage production of type II collagen. Front Bioeng Biotechnol doi: 10.3389/fbioe.2023.1179332 [Lenti-Pac™ HIV Expression Packaging Kit; GLuc-ON™ COL2A1 promoter-reporter clone]
- Park, J.H., et al. (2023). Statin prevents cancer development in chronic inflammation by blocking interleukin 33 expression. Res Sq doi: 10.21203/rs.3.rs-2318750/v1 [GLuc-ON™ promoter-reporter clone]
- Nong, Y., et al. (2023). UBE3A and transsynaptic complex NRXN1-CBLN1-GluD1 in a hypothalamic VMHvl-arcuate feedback circuit regulates aggression. bioRxiv doi: 10.1101/2023.02.28.530462 [GLuc-ON™ promoter-reporter clone]
- Pflug, K., et al. (2023). Transcriptional Induction of NF-kB-Inducing Kinase by E2F4/5 Facilitates Collective Invasion of Glioma Cells. Res Sq doi: 10.21203/rs.3.rs-2622363/v1 [GLuc-ON™ promoter-reporter clone]
- Yang, Y., et al. (2023). Graphene oxide/gallium nanoderivative as a multifunctional modulator of osteoblastogenesis and osteoclastogenesis for the synergistic therapy of implant-related bone infection. Bioact Mater doi: 10.1016/j.bioactmat.2022.07.015 [GLuc-ON™ promoter-reporter clone; Secrete-Pair™ Dual Luminescence Assay Kit]
- Zhang, J., et al. (2023). Naringenin in Si-Ni-San formula inhibits chronic psychological stress-induced breast cancer growth and metastasis by modulating estrogen metabolism through FXR/EST pathway. J Adv Res doi: 10.1016/j.jare.2022.06.006 [GLuc-ON™ promoter-reporter clone; Secrete-Pair™ Dual Luminescence Assay Kit]
- Cui, X., et al. (2023). Hepatocellular carcinoma-derived FOXO1 inhibits tumor progression by suppressing IL-6 secretion from macrophages. Neoplasia doi: 10.1016/j.neo.2023.100900 [GLuc-ON™ promoter-reporter clone; Secrete-Pair™ Gaussia Luciferase Assay Kit]
- Li, X., et al. (2023). Crosstalk between protein kinase C α and transforming growth factor β signaling mediated by Runx2 in intestinal epithelial cells. J Biol Chem doi: 10.1016/j.jbc.2023.103017 [GLuc-ON™ Tgfbr1 promoter-reporter clone]
- Luo, W., et al. (2023). Next-generation sequencing of 500 POI patients identified novel responsible monogenic and oligogenic variants. J Ovarian Res doi: 10.1186/s13048-023-01104-6 [GLuc-ON™ CYP19A1 promoter-reporter clone; HPRM30088-PG04]
- Zhu, L., et al. (2023). A Zeb1/MtCK1 metabolic axis controls osteoclast activation and skeletal remodeling. EMBO J doi: 10.15252/embj.2022111148 [GLuc-ON™ Ckmt1 promoter-reporter clone]
- Yu, C., et al. (2023). Flot2 acts as a novel mediator of podocyte injury in proteinuric kidney disease. Int J Biol Sci doi: 10.7150/ijbs.78945 [GLuc-ON™ Flot2 promoter-reporter clone]
- Togninalli, M., et al. (2023). Machine learning-based classification of dual fluorescence signals reveals muscle stem cell fate transitions in response to regenerative niche factors. NPJ Regen Med doi: 10.1038/s41536-023-00277-4 [MPRM15676-LvPM02]
- Kisler, K., et al. (2023). Anti-malaria drug artesunate prevents development of amyloid-β pathology in mice by upregulating PICALM at the blood-brain barrier. Mol Neurodegener doi: 10.1186/s13024-023-00597-5 [GLuc-ON™ promoter-reporter clone]
- Jeon, J., et al. (2023). Blockade of Activin Receptor IIB Protects Arthritis Pathogenesis by Non‐Amplification of Activin A‐ACVR2B‐NOX4 Axis Pathway. Adv Sci (Weinh) doi: 10.1002/advs.202205161 [GLuc-ON™ promoter-reporter clone]
- Wang, R., et al. (2023). SARS-CoV-2 restructures host chromatin architecture. Nat Microbiol doi: 10.1038/s41564-023-01344-8 [GLuc-ON™ IL6 promoter-reporter clone]
- Chen, X., et al. (2023). Interferon regulatory factor 1 (IRF1) inhibits lung endothelial regeneration following inflammation-induced acute lung injury. Clin Sci (Lond) doi: 10.1042/CS20220876 [GLuc-ON™ IRF1 promoter-reporter clone]
- Zheng, Y., et al. (2023). Chronic psychological stress promotes breast cancer pre-metastatic niche formation by mobilizing splenic MDSCs via TAM/CXCL1 signaling. J Exp Clin Cancer Res doi: 10.1186/s13046-023-02696-z [GLuc-ON™ CXCL1 promoter-reporter clone; Luc-Pair™ Duo-Luciferase Assay Kit 2.0]
