All-in-One™ qPCR Mix
All-in-One™ qPCR Mix
All-in-One™ qPCR Mix
产品介绍
All-in-One 编码基因验证引物
All-in-One qPCR mix试剂与基因特异引物在通用的qPCR反应条件下配套使用，即可获得可靠的实验数据。两种产品经共同开发并进行一体化优化，表现出了如您所期望的、无需任何高成本的全范围优势。
- 均一化的反应条件缩短实验设计时间，更早提交论文
- 高扩增效率和灵敏度（甚至是低丰度基因）意味着每次实验中的可靠定量
- 无非特异性扩增和二聚体，确保可重复性和数据可靠性
All-in-One qPCR mix使用高保真热启动酶、经过优化的反应缓冲液和高质量dNTPs，能够使低丰度RNA（cDNA）或DNA进行特异的、灵敏的扩增。
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| 扩增曲线 | 熔解曲线 | 标准曲线 |
图1.用梯度稀释的质粒DNA（5×106至5个分子）做标准曲线来评估All-in-One™ qPCR Mix的扩增效率和检测灵敏度。如扩增曲线和熔解曲线所示，All-in-One™ qPCR Mix可扩增低至5个分子的模板（质粒DNA）说明了扩增和检测的高灵敏度。
All-in-One 编码基因验证引物 
使用GeneCopoeia精确的qPCR试剂产品可消除繁冗的调整和优化过程。只需选择All-in-One验证引物即可开始。
All-in-One人、小鼠或大鼠的qPCR反应特异引物由专利算法设计，并为准确定量而进行了验证。验证结果包括熔解曲线，以保证扩增正确靶DNA序列（见图2.）。
与基于SYBR® Green I的All-in-One qPCR Mix试剂配套使用，All-in-One验证引物在qPCR定量实验中表现出可靠、可重复的高效性。
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| 扩增曲线 | |
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| 熔解曲线 | 验证结果的琼脂糖电泳 |
图2. 45对基因特异引物经过实验验证均产生单一扩增产物，融解曲线均呈单一锐锋，电泳条带均呈单一条带，且与目标基因相符。由10种人组织（肺、肝、睾丸、卵巢、脾、脑、胎盘、胰、心和乳腺）混合样本的总RNA经逆转录形成的cDNA作为引物验证的模板。qPCR反应由终浓度0.2 uM的引物和All-in-One qPCR Mix配合完成。使用Bio-Rad iQ5 qPCR仪器，在95°C预变性10分钟后，紧接着40个PCR循环（95°C变性10秒，60°C退火20秒钟，72°C延伸15秒），最后一个循环后设置72至95°C的熔解曲线检测程序。扩增曲线、熔解曲线和琼脂糖凝胶电泳图如图所示（验证的阳性结果见奇数泳道，阴性对照结果（NTC）见偶数泳道）。
注释：1.以上无非特异性扩增和二聚体的验证结果均建立于本公司配套试剂产品和反应体系之上。2.以上验证引物包括人、小鼠和大鼠，如需其他物种请咨询。
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发表的文章
- Chen, W., et al. (2025). Analysis of the effect of ALA-PDT on macrophages in footpad model of mice infected with Fonsecaea monophora based on single-cell sequencing. Open Med (Wars) DOI: 10.1515/med-2024-1132 [All-in-One™ qPCR Mix]
- Xia, H-F., et al. (2025). PCBP2-dependent secretion of miRNAs via extracellular vesicles contributes to the EGFR-driven angiogenesis. Theranostics DOI: 10.7150/thno.102391 [All-in-One™ qPCR Primer]
- Ding, J., et al. (2025). LncRNAs in serum-derived extracellular vesicles are potential biomarker and correlated with immune infiltration in gastric cancer. Front Immunol DOI: 10.3389/fimmu.2025.1533111 [All-in-One™ First-Strand cDNA Synthesis Kit, Cat. No. QP006; All-in-One™ qPCR Mix, Cat. No. QP001]
- Luo, H., et al. (2024). The miR-23a/27a/24 − 2 cluster drives immune evasion and resistance to PD-1/PD-L1 blockade in non-small cell lung cancer. Mol Cancer DOI: 10.1186/s12943-024-02201-w [All-in-One™ qPCR Primers for U6, has-miR-23a, has-miR-24-2 and has-miR-27a; All-in-One™ miRNA qRT-PCR Detection Kit 2.0]
- Zhu, P., et al. (2024). Adenosine deaminase acting on RNA 2 provides neuroprotection by activating Kv1.1 channels in a rat epilepsy model. Cytojournal DOI: 10.25259/Cytojournal_53_2024 [All-in-One™ qPCR Mix]
- Wang, X., et al. (2024). Exploring the therapeutic potential of puerarin on intervertebral disc degeneration by regulating apoptosis of nucleus pulposus cells. JOR Spine DOI: 10.1002/jsp2.70020 [All-in-One™ qPCR Primers for IL-1β, TNF-a, CASP3, CASP8 and BCL2]
- Khalifa, A.K., et al. (2024). New horizon of the combined BCG vaccine with probiotic and liraglutide in augmenting beta cell survival via suppression of TXNIP/NLRP3 pyroptosis signaling in Streptozocin–Induced diabetes mellitestype-1 in rats. Heliyon DOI: 10.1016/j.heliyon.2024.e38932 [All-in-One™ First-Strand cDNA Synthesis Kit; All-in-One™ qPCR Mix]
- Tu, H., et al. (2024). NOTCH2NLC GGC intermediate repeat with serine induces hypermyelination and early Parkinson’s disease-like phenotypes in mice. Mol Neurodegener DOI: 10.1186/s13024-024-00780-2 [All-in-One™ qPCR Mix, Cat. No. QP001]
- Khalifa, M.A.S., et al. (2024). Functional Characterisation of GmGASA1-like Gene in Glycine max (L.) Merr.: Overexpression Promotes Growth; Development and Stress Responses. Life (Basel) DOI: 10.3390/life14111436 [All-in-One™ qPCR Mix]
- Pant, A., et al. (2024). Stimulation of neutral lipid synthesis via viral growth factor signaling and ATP citrate lyase during vaccinia virus infection. J Virol DOI: 10.1128/jvi.01103-24 [All-in-One™ qPCR Mix]
- Li, F-X-Z., et al. (2024). Mechanism of cold exposure delaying wound healing in mice. J Nanobiotechnology DOI: 10.1186/s12951-024-03009-y [All-in-One™ qPCR Primer for Mouse snRNA U6, Cat. No. MmiRQP9001; All-in-One™ qPCR Primer against mature miRNA mmu-miR-423-3p, Cat. No. MmiRQP0491]
- Xia, C., et al. (2024). Identification of DLAT as a potential therapeutic target via a novel cuproptosis-related gene signature for the prediction of liver cancer prognosis. J Gastrointest Oncol DOI: 10.21037/jgo-24-609 [All-in-One™ qPCR Primer for ATP7A, LIPT1, DLAT, GAPDH; OMICSLINK™ Lentiviral shRNA clones targeting DLAT]
- Cui, D., et al. (2024). Upregulation of EFCAB7 after radiofrequency ablation promoting hepatocellular carcinoma metastasis and survival by regulating PARK7. Aging (Albany NY) DOI: 10.18632/aging.206073 [All-in-One™ qPCR Primers]
- Lin, X., et al. (2024). Endothelial cells derived extracellular vesicles promote diabetic arterial calcification via circ_0008362/miR-1251-5p/Runx2 axial. Cardiovasc Diabetol DOI: 10.1186/s12933-024-02440-7 [All-in-One™ miRNA qRT-PCR Detection Kit 2.0, Cat. No.AOMD-Q060; All-in-One™ qPCR primer for hsa_circ_0008362]
- Liu, J., et al. (2024). PPARγ agonist alleviates calcium oxalate nephrolithiasis by regulating mitochondrial dynamics in renal tubular epithelial cell. PLoS One DOI: 10.1371/journal.pone.0310947 [All-in-One™ qPCR Mix, Cat. No. QP001]
- Hon, J-X., et al. (2024). Exploring the Role of MicroRNAs in Progesterone and Estrogen Receptor Expression in Endometriosis. Biomedicines DOI: 10.3390/biomedicines12102218 [All-in-One™ miRNA First-Strand cDNA Synthesis Kit 2.0; All-in-One™ qPCR Primers for hsa-miR-199a-3p, hsa-miR-196a-5p, hsa-miR-144-3p, hsa-miR-1-3p, hsa-miR-146a-5p, hsa-miR-125b-5p, RNU6-2 and the internal control, Cat. No. HmiRQP0289, HmiRQP0284, HmiRQP0190, HmiRQP0044, HmiRQP0196, HmiRQP0096 and HmiRQP9001; miRNA Universal Adaptor PCR Primer, Cat. No. QP029]
- Shan, S-K., et al. (2024). Vascular wall microenvironment: Endothelial cells original exosomes mediated melatonin-suppressed vascular calcification and vascular ageing in a m6A methylation dependent manner. Bioact Mater DOI: 10.1016/j.bioactmat.2024.08.021 [BlazeTaq™ SYBR Green qPCR Master Mix (with ROX); All-in-One™ qPCR Primers]
- Yang, X., et al. (2024). Paclitaxel hyperthermia suppresses gastric cancer migration through MiR-183-5p/PPP2CA/AKT/GSK3β/β-catenin axis. J Cancer Res Clin Oncol DOI: 10.1007/s00432-024-05923-y [All-in-One™ miRNA qPCR Primer and Reference Primer for hsa-miR-183-5p, Cat. No. HmiRQP0244, HmiRQP9001]
- Xue, H., et al. (2024). The mechanism of NF-κB-TERT feedback regulation of granulosa cell apoptosis in PCOS rats. PLoS One DOI: 10.1371/journal.pone.0312115 [All-in-One™ qPCR Primer for Human IL-6, TERT and TNF-α, Cat. No. HQP009670, HQP018018, HQP018141]
- Yusuf, A.N.M., et al. (2024). Supraphysiological Dose of Testosterone Impairs the Expression and Distribution of Sex Steroid Receptors during Endometrial Receptivity Development in Female Sprague–Dawley Rats. Int J Mol Sci DOI: 10.3390/ijms251810202 [All-in-One™ qPCR Primer for Ar, Esr1, Pgr and GAPDH, Cat. No. RQP129826, RQP049019, RQP050618, RQP049537]
- Lei, L-M., et al. (2024). Cold exposure-induced plasma exosomes impair bone mass by inhibiting autophagy. J Nanobiotechnology. DOI: 10.1186/s12951-024-02640-z [All-in-One™ qPCR Primer]
- Jiang, N., et al. (2024). Overexpression of zinc finger DHHC-type containing 1 is associated with poor prognosis and cancer cell growth and metastasis in uterine corpus endometrial carcinoma. Aging (Albany NY). DOI: 10.18632/aging.205899 [All-in-One™ qPCR Primer for ZDHHC1]
- DeWolf, S.E., et al. (2024). Human pulmonary microvascular endothelial cells respond to DAMPs from injured renal tubular cells. Pulm Circ. DOI: 10.1002/pul2.12379 [All-in-One™ qPCR Primers]
- Zheng, L., et al. (2024). Single-cell sequencing analysis revealed that WDR72 was a novel cancer stem cells related gene in gastric cancer. Heliyon. DOI: 10.1016/j.heliyon.2024.e35549 [All-in-One™ qPCR Mix, Cat. No. QP001]
- Wu, Chan., et al. (2024). Pyroptosis and mitochondrial function participated in miR-654-3p-protected against myocardial infarction. Cell Death Dis. DOI: 10.1038/s41419-024-06786-4 [All-in-One™ miRNA First-Strand cDNA Synthesis Kit; mature miR-654-3p qPCR primers (MmiRQP0764F); SnRNA U6 qPCR primers(MmiRQP9002)]
- Chen, N., et al. (2024). Sleep fragmentation exacerbates myocardial ischemia‒reperfusion injury by promoting copper overload in cardiomyocytes. Nat Commun. DOI: 10.1038/s41467-024-48227-y [All-in-One™ qPCR Mix, Cat. No. QP001]
- Zhang, T., et al. (2024). Testosterone deficiency worsens mitochondrial dysfunction in APP/PS1 mice. Front Aging Neurosci. DOI: 10.3389/fnagi.2024.1390915 [All-in-One™ qPCR Mix, Cat. No. QP001]
- Zhan, T., et al. (2024). CRISPR-based gene expression platform for precise regulation of bladder cancer. Cell Mol Biol Lett. DOI: 10.1186/s11658-024-00569-7 [All-in-One™ qPCR Mix, Cat. No. QP001]
- Xiang, L., et al. (2024). Sorbitol Destroyed Intestinal Microfold Cells (M Cells) Development through Inhibition of PDE4-Mediated RANKL Expression. Mediators Inflamm. DOI: 10.1155/2024/7524314 [All-in-One™ qPCR Mix]
- Dong, Z., et al. (2024). Regulation of mitochondrial network architecture and function in mesenchymal stem cells by micropatterned surfaces. Regen Biomater. DOI: 10.1093/rb/rbae052 [All-in-One™ qPCR Mix, Cat. No. QP001]
- Velankar, K.Y., et al. (2024). Fibril-Guided Three-Dimensional Assembly of Human Fibroblastic Reticular Cells. ACS Appl Bio Mater. DOI: 10.1021/acsabm.4c00331 [All-in-One™ qPCR Mix, Cat. No. QP001]
- Dare, A., et al. (2024). Surfactant protein A promotes western diet-induced hepatic steatosis and fibrosis in mice. Sci Rep. DOI: 10.1038/s41598-024-58291-5 [All-in-One™ qPCR Mix]
- Lu, Y., et al. (2024). Characterization of the biological and transcriptomic landscapes of bone marrow-derived mesenchymal stem cells in patients with multiple myeloma. Cancer Cell Int. DOI: 10.1186/s12935-024-03308-2 [SureScript™ First-strand cDNA Synthesis kit; All-in-One™ qPCR Mix]
- Wang, X., et al. (2024). Macrophage-enriched Sectm1a promotes efficient efferocytosis to attenuate ischemia/reperfusion-induced cardiac injury. JCI Insight. DOI: 10.1172/jci.insight.173832 [All-in-One™ qPCR Mix, Cat. No.QP005]
- Li, X., et al. (2024). Apoptotic extracellular vesicles restore homeostasis of the articular microenvironment for the treatment of rheumatoid arthritis. Bioact Mater. DOI: 10.1016/j.bioactmat.2023.11.019 [All-in-One™ First-Strand cDNA Synthesis Kit; All-in-One™ qPCR Mix]
- Creus-Muncunill, J., et al. (2024). TYROBP/DAP12 knockout in Huntington’s disease Q175 mice cell-autonomously decreases microglial expression of disease-associated genes and non-cell-autonomously mitigates astrogliosis and motor deterioration. J Neuroinflammation. DOI: 10.1186/s12974-024-03052-4 [All-in-One™ qPCR Mix]
- Xue He, et al. 2024.PU.1 alleviates the inhibitory effects of cigarette smoke on endothelial progenitor cell function and lung-homing through Wnt/β-catenin and CXCL12/CXCR4 pathways. Tob Induc Dis.DOI:10.18332/tid/174661[All-in-One™ qPCR Mix]
- Yuehong Chen, et al. 2024.microRNA-622 upregulates cell cycle process by targeting FOLR2 to promote CRC proliferation. BMC Cancer.DOI:10.1186/s12885-023-11766-6[snRNA U6 qPCR Primer]
- Denis Voronin, et al. 2024.Repurposed Drugs That Activate Autophagy in Filarial Worms Act as Effective Macrofilaricides. Pharmaceutics.DOI:10.3390/pharmaceutics16020256[All-in-One™ qPCR Mix, Cat. No.QP001]
- Mei Zhou, et al. 2024.The dynamic role of nucleoprotein SHCBP1 in the cancer cell cycle and its potential as a synergistic target for DNA-damaging agents in cancer therapy. Cell Commun Signal.DOI:10.1186/s12964-024-01513-0[All-in-One™ qPCR Mix；All-in-One™ First-Strand cDNA Synthesis Kit]
