– 更稳定，更灵活
Luc-Pair™ 双荧光素酶检测试剂盒 2.0（Firefly and Renilla）
Luc-Pair™ Duo-Luciferase Assay Kit 2.0（双荧光素酶检测试剂盒 2.0）是由第一代 LucPair 双荧光素酶检测试剂盒改进所得，具有更高的稳定性和使用灵活性，可搭配使用 96 孔板，方便、高效检测萤火虫和海肾荧光素酶活性。由于其稳定性增强，本试剂盒是用于检测 miRNA 对靶基因抑制效率的理想工具。
产品特点
- 稳定性更高
- 适用范围更灵活
- 背景更低，数据更准确
- 在广泛动态范围内具有高灵敏度
- 适用于所有哺乳动物细胞的荧光素酶检测
- 实验重复性好
- 操作简单、价格实惠
- 适用于高通量筛选
产品应用及优势
- 本产品具有极高稳定性。添加底物 30 分钟后，荧光素酶活性仍保持在 80% 以上；
- 本产品是研究 miRNA 前体表达克隆和 miRNA 3’UTR 克隆之间相互作用的理想工具；
- 操作简便快捷，短时间即可获得检测结果；
- 本检测方法可搭配微孔板（如 96 孔板）使用，节省实验时间和实验经费。
图 1. 荧光信号的稳定性比较。以 GeneCopoeia pEZX-MT06 miRNA 报告载体转染 HEK 293 细胞，转染 48 小时后分别检测荧光信号，效果如上图所示。从图中可知，GeneCopoeia 试剂盒（图A）的 Fluc 及 Rluc 均在 60 分钟内保持信号稳定，竞争对手品牌的试剂盒（图B）荧光信号在前 30 分钟已出现较大波动。
图 2. 转入细胞的载体数量与荧光激发量的线性关系比较。以 GeneCopoeia pEZX-MT06 miRNA 报告载体转染 HEK 293 细胞，转染48小时后，按梯度取不同细胞数量，溶解细胞后分别检测荧光信号，效果如上图所示。从图中可知，GeneCopoeia 试剂盒（红色）检测所得结果中，Fluc 和 Rluc 的线性关系比较好，竞争对手品牌的试剂盒（蓝色）检测所得结果的线性关系相对较差。
载体选择
GeneCopoeia 提供萤火虫荧光素酶（Firefly luciferase）报告载体，可供广大研究者自由操作；只需将目的片段插入报告载体，即可应用荧光素酶检测试剂。 点击载体名称可查看载体图谱信息。
| 货号 | 载体 | 启动子 | 报告基因 | 示踪基因 | 筛选标记 | 抗性 | 载体类型 |
| ZX001 | pEZX-FR01 | N/A* | Firefly luciferase (hLuc) | Renilla luciferase (Rluc) | Puromycin | Kan | Non-viral |
| ZX002 | pEZX-FR02 | SV40 | Firefly luciferase (hLuc) | Renilla luciferase (Rluc) | N/A* | Amp | Non-viral |
| ZX003 | pEZX-FR03 | miniCMV | Firefly luciferase (hLuc) | Renilla luciferase (Rluc) | Puromycin | Kan | Non-viral |
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